Matrix Metalloproteinase Protocols

Matrix Metalloproteinase Protocols

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From the simple discovery in 1962 that resorbing tadpole tail expressed an enzyme (MMP) that could degrade collagen gels, matrix metalloproteinase (MMP) research has advanced to discover more than twenty distinct vertebrate MMPs and four specific inhibitors (TIMPS), a veritable family of enzymes involved in many physiological and pathological processes. In Matrix Metalloproteinase Protocols, leading experts detail proven laboratory techniques for the study of MMPs. The methods include those for the expression and purification of MMPs and TIMPs, for the detection of MMPs and TIMPs at both the protein and mRNA levels, and for the assay of MMP and TIMP activities in a wide variety of circumstances. Each method includes step-by-step instructions and notes on variant applications and pitfalls to avoid. A selective overview of the MMP arena spells out where the field has been, where it is, and where it is going. Comprehensive and highly practical, Matrix Metalloproteinase Protocols brings together the long and hard-earned experience of master experimentalists that will allow not only novices to get up to speed quickly, but also add to the repertoire of successful techniques in expert laboratories.... lowledgments ;rate fully acknowledge David Miles of Invitrogen for allowing the repro- on of some of their manual protocols and vector maps. ... a#39;regg, J. M., Vedvick, T. S., and Raschke, W. C. (1993) Recent advances in the xpression of foreign genes in Pichia pastoris. ... Gene 190, 55-62. losenfeld, S. A., Ross, O. H., Hillman, M. C, Corman, J. I., and Dowling, R. L. 1996) Production and purification ofanbsp;...

Title:Matrix Metalloproteinase Protocols
Author: Ian M. Clark
Publisher:Springer Science & Business Media - 2001-01-01

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